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rat asic2a antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rat asic2a antibody
    Rat Asic2a Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+asic2a+antibody/pm27934921-296-46-49
    Average 86 stars, based on 1 article reviews
    rat asic2a antibody - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception.
    Article Snippet: Equal amounts of protein (20 μ g) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β -actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception
    Article Snippet: Equal amounts of protein (20 μg) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β-actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.

    Saline:

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception.
    Article Snippet: Equal amounts of protein (20 μ g) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β -actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception
    Article Snippet: Equal amounts of protein (20 μg) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β-actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.

    Incubation:

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception.
    Article Snippet: Equal amounts of protein (20 μ g) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β -actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception
    Article Snippet: Equal amounts of protein (20 μg) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β-actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.

    Purification:

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception.
    Article Snippet: Equal amounts of protein (20 μ g) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β -actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.

    Article Title: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception
    Article Snippet: Equal amounts of protein (20 μg) were resolved in 10% SDS-polyacrylamide gels and transferred to a polyvinylidene fluoride membrane (Millipore Corp., Bedford, MA, USA) for Western blot analysis. .. After blocking the membranes with 5% nonfat milk in Tris buffer saline Tween 20 (TBST: 20 mM Tris, pH 7.6, 120 mM NaCl, 0.1% Tween), they were incubated with a 1:1000 dilution of the specific purified rabbit anti rat ASIC1 antibody (Alomone Lab, Israel), rabbit anti rat ASIC2a antibody (Alomone Lab, Israel) or mouse anti rat anti-β-actin antibody (Sigma-Aldrich, USA) at 4 °C for 12 h, followed by extensive washes with TBST. .. A 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG or anti-mouse IgG (Beyotime Institute of Biotechnology, China) was added and membranes were incubated for 1 h at room temperature.



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